SRA

Organophosphate pesticide, dichlorvos is one of the most widely used pesticides in developing countries for controlling domestic insects/pests. As dichlorvos is difficult to degrade without intervention, residues regarded as persistent organic pollutants threaten the environment, requiring more energy to remediate the same. Moreover, prolonged exposure to them exhibits genotoxic, neurological, and immunopathologic effects. Therefore, eco-friendly biodegradation is a useful intervention against excessive pesticide use and its environmental abuse. This study investigates the degrading potential and kinetics of microbial isolates against dichlorvos (2, 2- dichlorovinyl dimethyl phosphate). Isolation was primarily carried in the Minimal Salt Medium (broth) supplemented with pesticide at low concentrations. Pesticide-degrading bacterial isolates were purified on MSM agar supplemented with DDVP (dichlorovos) as a sole carbon source. Colonies were then processed to decipher identify first with morphological, cultural, biochemical and 16s rRNA sequencing. These bacterial strains were isolated from farmland exposed to dichlorvos for three consecutive farming sessions and successfully identified as Klebsiella pneumoniae and Enterobacter cloacae based on morphological, cultural, biochemical and 16S rRNA sequencing analysis.